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    科学领域:

    • 生物医学工程 生物医学工程
    • 眼科医生 眼科 眼科
    • 药物发现 药物发现 药物发现

    背景情况:

    • 高通量查 (HTS) 对于药物发现和生物研究至关重要.
    • 现有的HTS方法往往缺乏详细组织分析所需的分辨率或自动化.
    • 光学连贯断层扫描 (OCT) 提供非侵入性,高分辨率成像,但需要适应HTS.

    研究的目的:

    • 开发和验证一个完全自动化的OCT成像系统,用于高通量查ex vivo组织培养物.
    • 整合先进的自动化和深度学习,以实现稳健高效的数据采集和分析.
    • 证明系统在疾病模型中评估组织对治疗的反应的能力.

    主要方法:

    • 开发一个定制的OCT系统,配有用于自动化样品处理和连续成像的机动平台.
    • 实现基于变压器的深度学习算法,用于自动组织细分和分析.
    • 验证使用从视网膜退行症小鼠模型的ex vivo视网膜爆炸培养物.

    主要成果:

    • 自动化的OCT系统成功地对组织样本进行了高通量,连续的成像.
    • 深度学习细分提供了强大的,一致的和高效的定量读数.
    • 该系统展示了对视网膜扩展剂对治疗反应的快速,可靠和公正的评估.

    结论:

    • 开发的自动化OCT系统显著提高了ex vivo组织查能力.
    • 这项技术有望加速药物发现和其他需要高通量组织分析的研究领域.
    • 该系统提供了一个强大的,非侵入性的工具,用于全面评估基于组织的测试.