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In vitro Monitoring of Extracellular pH in Real-Time
Published on: June 3, 2021
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在肠道细菌感染期间,病原体和宿主都能动态地适应肠道沿线的pH反应
Sarah E Woodward1,2, Laurel M P Neufeld1, Jorge Peña-Díaz1,2
1Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
PLoS biology
|August 15, 2024
概括
杆菌适应肠道pH值,改变毒性和生长. 胃酸触发反应性氧物种 (ROS),但适应性增强了生存,揭示了宿主-病原体pH共同演变.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 传染性疾病 传染性疾病
背景情况:
- 肠道病原体适应不同的肠道环境.
- 胃肠道的pH值因地区而异,影响病原体的行为.
研究的目的:
- 研究Citrobacter rodentium对胃肠道pH的反应.
- 了解病原体的适应和宿主病原体的共同进化.
主要方法:
- 在不同pH值下评估C. rodentium的基因表达和形态.
- 在胃pH下测量反应性氧物种 (ROS) 积累.
- 分析了宿主基因表达和感染后的胃素水平.
主要成果:
- 小肠的pH (4.4-4.8) 诱导了毒性和附着性.
- 较高的pH值支持了C. rodentium的生长.
- 胃pH通过ROS积累抑制了生长.
- 感染导致胃pH值降低,宿主胃蛋白增加.
- 酸耐受性反应 (ATR) 增强了胃酸存活率.
结论:
- 细菌和宿主的pH反应对于肠道殖民和共同进化至关重要.
- 感染后宿主胃的pH值变化可能会限制病原体的摄入.
- 了解pH适应是控制肠道感染的关键.
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