构建与压力相关的LncRNAs肺腺癌的肺内质网膜特征
Kai Chen1, Peiling Dai2, Lizhong Gu1
1The Department of Cardiovascular and Thoracic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
The journal of gene medicine
|August 15, 2024
概括
这项研究确定了七种与内质网膜压力相关的长非编码RNA (lncRNAs) 作为肺腺癌 (LUAD) 的潜在预后标志物,提供了新的治疗见解.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 细胞内膜网膜应激 (ERS) 是一种潜在的癌症治疗策略.
- 长非编码RNAs (lncRNAs) 与癌症进展和预后有关.
- 在肺腺癌 (LUAD) 中与ERS相关的lncRNAs的预后作用仍然未被探索.
研究的目的:
- 在肺腺癌 (LUAD) 中识别和验证预后ERS相关的长非编码RNA (lncRNAs).
- 开发基于ERS相关的lncRNAs的LUAD预后预测模型.
- 阐明涉及这些lncRNAs的潜在分子机制.
主要方法:
- 使用TCGA和GEO数据库进行LUAD表达数据 (mRNA,miRNA,lncRNA).
- 确定了与ERS相关的差异表达lncRNAs (DELs),并使用Cox回归构建了预后特征.
- 构建了一个lncRNA-miRNA-mRNA网络并进行基因丰富分析.
- 使用定量实时聚合酶链反应 (qRT-PCR) 验证的 lncRNA表达.
主要成果:
- 确定了30个ERS-DEL,并开发了7个lncRNA签名 (AF131215.2,LINC00472,LINC01352,RP1-78O14.1,RP11-253E3.3,RP11-98D18.9,SNHG12) 用于LUAD预后.
- 高风险组的基因与mRNA结合有关,而低风险组的基因则专注于蛋白质定位到乳毛.
- 建立了一个全面的lncRNA-miRNA-mRNA网络,涉及七个标志性lncRNA,23个miRNA和128个mRNA.
- qRT-PCR证实了七种预后 lncRNAs 的表达模式.
结论:
- 开发了一种新的预后特征,包括七个与ERS相关的lncRNA用于LUAD.
- 这种特征为与ERS相关的lncRNAs在LUAD进展中的作用提供了新的见解.
- 这些发现可能有助于开发针对LUAD的向疗法.
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