基于二氧化粉和高光强度的液化床光生物反应器改善了微藻收获,营养物质的去除和脂质的积累:性能和微观机制
Haolian Xu1, Hong Wang1, Xiankai Wang2
1State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Water research
|August 15, 2024
概括
使用无氧消化物培养微藻可以使用具有藻载体的新型流化床光生物反应器 (FBPBR) 进行增强. 这种方法提高了生物质度,脂质含量和营养物质去除效率.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 环境科学与工程环境科学与工程
- 藻类生物技术 藻类生物技术
背景情况:
- 使用无氧消化剂种植微藻为生物质生产和环境修复提供了双重好处.
- 传统的悬浮微藻培养系统的生物质度低,收获成本高.
研究的目的:
- 开发和评估一种新型的流化床光生物反应器 (FBPBR),使用藻粉来培养Scenedesmus quadricauda.
- 评估FBPBR在处理稀释无氧消化剂和提高微藻生物质生产和质量的效率.
主要方法:
- 使用二氧化粉作为载体材料构建流化床光生物反应器 (FBPBR).
- 在FBPBR中使用稀释无氧消化剂在不同光强度下培养Scenedesmus quadricauda.
- 分析微藻生物质度,沉效率,细胞外聚合物物质 (EPS) 特性,脂质含量和营养物质去除,使用转录组分析和生物化学分析.
主要成果:
- 优化的二氧化载体剂量 (750 mg/L) 将微藻生物质度提高到 1.58 g/L.
- 增加的光强度 (200μmol/m2/s) 将生物质进一步提升到1.84g/L,沉效率达到93.58%,这是由于改善了EPS分泌和特性.
- 二氧化和高光显著增加了脂质含量至60.37%和营养物质去除率 (93.24%NH4+-N,96.86%TP),得到了转录组数据的支持,显示了高调的脂质合成和营养物质代谢基因.
结论:
- 基于二氧化的FBPBR是一种有效的系统,可以增强微藻生物质的产生,脂质的积累和从无氧化消化物中去除营养.
- 二氧化载体和优化的光照条件减轻无氧消化压力,促进抗氧化防御,并改善光合作用在Scenedesmus quadricauda.
- 这项研究为生物燃料和废水处理应用开发高效的微藻培养系统提供了宝贵的见解.
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