相关实验视频

Updated: May 3, 2026

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Published on: July 13, 2017

在Meretrix meretrix中对C型乳素基因的分子鉴定和功能特征

Lu Zhang¹, Cun Wei¹, Ying Guo²

¹Hainan Key Laboratory of Tropical Aquatic Germplasm, Sanya Oceanographic Institution, Ocean University of China, Sanya, 572024, China; MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China.

Fish & shellfish immunology

|August 15, 2024

概括

研究人员在硬中发现了一种新的C型讲蛋白 (CTL),MmCTL4,它在天生的免疫力中起作用. 这种模式识别受体 (PRR) 呈现出凝血活性,并有助于防御细菌和真菌病原体.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
生物化学生化学

背景情况:

C型讲蛋白 (CTLs) 是依赖的免疫因子,参与病原体的识别和防御.
硬 (Meretrix meretrix) 是重要的海洋无脊椎动物,具有复杂的先天免疫系统.

研究的目的:

为了识别和表征一种来自Meretrix meretrix的新型C型乳清素 (MmCTL4).
研究MmCTL4.4的免疫功能和病原体识别能力.

主要方法:

克隆cDNA和分析MmCTL4.4的序列.
再组合蛋白表达和净化MmCTL4.
SYBR绿色实时PCR用于基因表达分析.
血液凝结试验用于评估病原体结合活性.

主要成果:

一个新的CTL,MmCTL4,被识别为具有特征的碳水化合物识别域 (CRD).
在刺激Vibrio splendidus后,血细胞和肝中MmCTL4表达显著增加.
重组的MmCTL4 (rMmCTL4) 显示出对各种细菌 (大肠杆菌,B. subtilis,S. aureus) 和真菌 (P. pastoris) 的血液凝结活性.
聚合活性被特定的碳水化合物和脂多糖抑制,表明特定的分子识别.

结论:

在硬天生的免疫系统中,MmCTL4作为模式识别受体 (PRR) 起作用.
MmCTL4通过识别和潜在地中和微生物病原体,有助于宿主防御.
这项研究增强了对CTLs在无脊椎动物免疫反应中的作用的理解.

关键词:

在C型乳清中,C型的乳清含有这是天生的免疫力.梅雷特里克斯梅雷特里克斯梅雷特里克斯模式识别受体是一种模式识别受体.在QPN和WSD图案中,有QPN和WSD图案.

更多相关视频

The Lambda Select cII Mutation Detection System

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...