菌素的采矿和多方面的应用,用于对抗Vibrio parahaemolyticus
Jing Liu1, Qian Wu1, Pradeep K Malakar1
1College of Food Science and Technology, Shanghai Ocean University, 999# Hu Cheng Huan Road, Shanghai 201306, China; International Research Center for Food and Health, Shanghai Ocean University, Shanghai 201306, China.
Food research international (Ottawa, Ont.)
|August 15, 2024
概括
菌素有效地对抗食物传播的病原体Vibrio parahaemolyticus. 这些蛋白质有望减少食品和环境中的细菌污染,为抗生素提供替代品.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品安全 食品安全
背景情况:
- 维亚血溶菌是海鲜和水中的一个重要的食物传播病原体.
- 在V. parahaemolyticus的抗生素耐药性需要替代的控制策略.
- 菌素素为病原体控制提供了一种安全有效的替代方案.
研究的目的:
- 系统地评估菌体 lysins 对它们对 Vibrio 物种的有效性.
- 描述选择的Vibrio菌素 lysins酶活性的最佳条件.
- 评估这些素在控制V. parahaemolyticus在各种环境中的潜力.
主要方法:
- 来自NCBI数据库的37种菌素的生物信息分析.
- 三种选择的素的表达和净化:Lyz_V_pgrp,Lyz_V_prgp60和Lyz_V_zlis.
- 在不同的条件下 (温度,盐,pH,透剂) 进行酶活性测定.
- 在体外对63种V. parahaemolyticus分离物的测试,包括抗生素协同作用和生物膜破坏.
- 在污染食品表面和环境样本上的lysin有效性的现场评估.
主要成果:
- 三种素 (Lyz_V_pgrp,Lyz_V_prgp60,Lyz_V_zlis) 在45°C,800mM NaCl和pH 8-10时表现出最佳活性,这种活性被膜透剂增强.
- 素有效抑制了V. parahaemolyticus分离物,逆转了抗生素耐药性,与抗生素协同作用,并破坏了生物膜 (>90%的减少).
- 在食品表面 (1.06-1.29日志CFU/cm2) 和环境样本 (0.87CFU/mL) 上观察到细菌负载的显著减少.
结论:
- 菌素素显示出对抗Vibrio parahaemolyticus的强有力的抗菌活性.
- 这些素有效地减少了食品表面和环境矩阵中的污染.
- 菌素素为控制V. parahaemolyticus提供了一种有希望和多功能的方法.
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