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使用元基因组学识别从无菌中引起的致病真菌,然后在现场进行杂交

Hiroya Oki^1,2, Ryotaro Niwa^3,4, Somboonthum Pranee⁵

¹Department of Infection Metagenomics, Research Institute for Microbial Diseases (RIMD), Osaka University, Osaka, Japan.

Access microbiology

|August 16, 2024

概括

诊断侵袭性真菌感染可能是具有挑战性的. 先进的基因组测序和现场杂交成功地在患有急性髓性白血病的患者中识别了*Trichosporon asahii*,即使标准培养失败了.

科学领域:

医学真菌学医学真菌学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

侵入性真菌感染 (IFI) 需要及时诊断才能有效治疗.
像显微镜和血液培养等标准诊断方法往往无法在无菌部位识别致病真菌.
即使使用抗真菌预防,突破性的IFI也可能发生.

研究的目的:

报告一个突破性入侵性三胞炎病例在免疫受损患者.
突出先进的分子技术的实用性,用于识别常规方法不足时的真菌病原体.

主要方法:

在真菌基因组的内部转录间隔器1 (ITS1) 区域进行深度测序.
在现场混合化使用针对26S核糖体RNA (rRNA) 的DNA探针.

主要成果:

血液培养对真菌生长有负面影响.
对肝脏活检和腹的显微镜检查显示了碎片化的真菌,但无法识别.
深度测序确定了潜在的真菌候选者.
在现场杂交证实了组织样本中存在*Trichosporon asahii*.

结论:

先进的基因组和分子技术对于诊断具有挑战性的侵入性真菌感染至关重要.
在这种情况下, *Trichosporon asahii* 被确定为突破性三性的致病原体.
这些方法为IFI提供了有价值的回顾性诊断能力.

关键词:

在AML,AML就是AML.国家储备系统 (NGS)在T. asahii的故事中.甲基基因组 (metagenome) 是一个基因组.

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