斯里帕克复合体控制着基因组的稳定性,性发育和毒性
Patricia P Peterson1, Jin-Tae Choi2, Ci Fu3
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA.
bioRxiv : the preprint server for biology
|August 16, 2024
概括
在Cryptococcus neoformans STRIPAK复合体对于保持基因组稳定性和调节真菌生长,性发育和毒性至关重要. 突变会破坏这些过程,影响病原体的适应性和宿主相互作用.
科学领域:
- 分子和细胞生物学分子和细胞生物学
- 菌类学 菌类学是指菌类学.
- 遗传学 是一个遗传学.
背景情况:
- 欧核生物的氨酸/氨酸蛋白酸酶PP2A是一种异构三分子酶.
- B'亚单元 (状蛋白) 与状蛋白相互作用的酸酶和激酶 (STRIPAK) 复合体相互作用.
- 在Cryptococcus neoformans中存在STRIPAK组件的正义词.
研究的目的:
- 在Cryptococcus neoformans中对STRIPAK组件Pph22,Far8和Mob3进行功能性表征.
- 研究C. neoformans STRIPAK复合体在基因组稳定性,生长和毒性中的作用.
主要方法:
- 全基因组测序以确定STRIPAK子单元中的突变.
- 删除突变的功能性特征 (pph22Δ,far8Δ,mob3Δ).
- 在小鼠模型中分析了哈普洛缺陷,生长缺陷,性差异化和毒性.
主要成果:
- 在STRIPAK子单元的突变导致形积分增加,表明在基因组稳定性中的作用.
- STRIPAK组件对于细胞生长,分泌,交配和性差异化至关重要.
- PPH22或FAR8的损失会损害生长,耐热性和毒性,而mob3Δ会增强耐热性,囊生产,黑色化和高毒性.
结论:
- 新型菌的STRIPAK复合体对基因组稳定,植物生长和性发育至关重要.
- 在C. neoformans.中,STRIPAK的成分差异性调节毒性和环境适应性.
- 了解STRIPAK的功能,可以了解真菌病原和潜在的治疗点.
相关概念视频
01:23Cystic Fibrosis: Pathogenesis
199
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
199
01:57Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
02:41Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
02:53CRISPR and crRNAs
16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K
00:46Genomic DNA in Prokaryotes
43.7K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.7K
01:32Replication in Prokaryotes
24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.8K