相关实验视频

Updated: Jun 16, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

多种类型的冷EM校准和工作流验证标准

Daija Bobe¹, Mykhailo Kopylov¹, Jessalyn Miller¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA.

bioRxiv : the preprint server for biology

|August 16, 2024

概括

研究人员现在有一个新的校准标准用于低温电子显微镜 (cryoEM). 这个测试样本有助于优化分辨率和评估结构生物学仪器性能.

科学领域:

结构生物学是结构生物学.
生物物理学的生物物理.

背景情况:

低温电子显微镜 (cryoEM) 是一种用于确定分子结构的强大技术.
既定方法往往缺乏标准化的校准样本,阻碍了性能评估.

研究的目的:

为了引入一种新的冷EM校准样本.
为验证冷EM管道和方法提供标准化工具.

主要方法:

开发了一种兼容的宏分子混合物作为单个试验样本.
设计了样本以优化分辨率和多点性能评估.

主要成果:

校准样本可以同时评估仪器性能,数据质量和图像处理工作流.
为验证和比较冷EM技术提供了参考点.

结论:

这种冷EM校准样本满足了对标准化生物试验样本的需求.
促进可靠的验证和对冷EM数据采集和分析的基准测试.

更多相关视频

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...