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在体内,抗体标记路径和光体决定了标记的效率,灵敏度和寿命

Natalie B Hagan¹, Charles Inaku¹, Nikesh Kunder²

¹Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

bioRxiv : the preprint server for biology

|August 16, 2024

概括

用特定光体注射抗CD45.2抗体的腹腔内注射延长了白细胞标记时间,从而更好地追踪罕见细胞迁移事件,特别是进入瘤.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

白细胞迁移对于对宿主进行感染和癌症的监测至关重要.
静脉注射 (IV) 抗CD45.2抗体标签可以追踪白细胞,但其窗口有限.
在跟踪罕见的白细胞迁移事件中存在挑战,因为标记时间狭窄.

研究的目的:

研究延长抗CD45.2抗体在白细胞追踪中的活性标记时间的方法.
评估抗体输送途径 (IV vs. 腹腔内) 和光体选择对标签动学的影响.
优化抗体标记,以便更好地检测白细胞迁移,特别是进入瘤.

主要方法:

对抗CD45.2抗体的IV和腹腔内 (IP) route的比较.
随着时间的推移,循环无结合抗体水平的动态分析.
用体外和体内测试评估各种光体 (例如,Alexa Fluor 647,Brilliant Blue 700) 的标记灵敏度.
使用优化标记策略,追踪白细胞迁移到瘤中的情况.

主要成果:

与IV给药相比,IP抗CD45.2抗体的给药导致持续的未结合的抗体水平.
明亮的蓝色700 (BB700) 和亚历克萨647 (AF647) 显示了高的标签敏感性.
IP anti-CD45.2 BB700抗体使白细胞连续标记超过6小时.
IP抗CD45.2 BB700促进了大约7倍多的瘤透CD8+T细胞的识别.

结论:

改变抗体注射途径和光剂可显著延长抗CD45.2抗体标签的持续时间.
与BB700-结合抗CD45.2抗体的IP管理对于在长时间内跟踪白细胞缓慢迁移是优越的.
这种优化的方法提高了识别最近迁移的白细胞的能力,包括瘤特异性T细胞,改善了对癌症免疫反应的理解.

关键词:

血液邻器官血液邻器官在体内标签的标签.注射的途径注射的途径.在T细胞迁移过程中,

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