microRNA-1通过Pyruvate代谢调节骨肌肉中的代谢灵活性
Ahmed Ismaeel1,2, Bailey D Peck3, McLane M Montgomery4
1Department of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.
bioRxiv : the preprint server for biology
|August 16, 2024
概括
骨肌中的microRNA-1 (miR-1) 损失会引发代谢变化,促进糖解并阻碍运动表现. 这突显了miR-1在调节肌肉能量代谢中的关键作用.
科学领域:
- 分子生物学分子生物学
- 骨肌肉生理学 骨肌肉生理学
- 代谢调节 代谢调节 代谢调节
背景情况:
- 微RNA-1 (miR-1) 在成年骨肌中非常丰富.
- 在成年骨肌代谢中,miR-1的确切功能尚不完全理解.
研究的目的:
- 为了阐明miR-1在成年骨肌中的功能作用.
- 确定miR-1向基因,并了解其在肌肉代谢中的调节机制.
主要方法:
- 产生一种可诱导的,骨肌特异性的miR-1淘汰赛 (KO) 鼠标模型.
- 整合RNA测序 (RNA-seq) 和阿尔戈诺特2增强交联和免疫沉降测序 (AGO2 eCLIP-seq) 数据.
- 综合的生物能量,代谢,蛋白质和代谢表型.
主要成果:
- 失去miR-1诱导了类似癌症的代谢重编程,增加了pyruvate kinase肌肉异酶M2 (PKM2) 和促进糖解.
- miR-1 缺乏导致了由于酸盐氧化抵抗的代谢不灵活性.
- 在小鼠和C.小鼠中,miR-1遗传损失损害了耐力运动表现. 伊莱根斯 (elegans) 是一个词.
结论:
- miR-1 是成年骨肌代谢的关键调节剂,控制糖解和酸盐氧化.
- 在缩期间观察到的miR-1的下调,通过代谢重编程支持肌肉生长.
- 确定了一种由miR-1介导的骨肌肉代谢调节的新型后翻译机制.
关键词:
这里是C. elegans.华堡效应是什么? 华堡效应有氧糖解有氧糖解.这就是eCLIP-seqq.过度缩的过度缩是一个问题.代谢生物组的代谢生物组这是一个微型RNA.蛋白质组学 蛋白质组学耐力运动是一种抵抗运动.更多相关视频
09:40Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
Published on: January 19, 2017
11.7K
08:01Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
Published on: May 16, 2021
5.7K
相关概念视频
01:20Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
00:56What is Glycolysis?
164.1K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
164.1K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:15Pyruvate Oxidation
158.6K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
158.6K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K