生产性mRNA染色质逃逸是由PRMT5SNRPBB甲基化促进的
Joseph D DeAngelo1,2, Maxim I Maron1,2,3, Jacob S Roth1
1Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
bioRxiv : the preprint server for biology
|August 16, 2024
概括
蛋白质氨酸甲基转移酶5 (PRMT5) 控制特定mRNA的处理和拼接,使其从染色质中释放出来. 这确保了适当的RNA输出,影响了基因表达调节.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 已知蛋白质氨酸甲基转移酶5 (PRMT5) 通过对称二甲基化来调节RNA剪接和转录.
- 将PRMT5的拼接调节与转录输出联系在一起的确切机制尚不清楚.
研究的目的:
- 阐明PRMT5将RNA拼接与转录输出的机制.
- 确定和描述一种由PRMT5.5调节的新型mRNA类.
主要方法:
- 新生的和总的转录组学.
- 斯派克控制的分离细胞转录组学.
- 总细胞和分离细胞蛋白质组学
- 在PRMT5抑制和淘汰研究中.
- 对氨酸突变SNRPB的研究
主要成果:
- PRMT5的活性促进了一种新型mRNA类的染色质逃逸,称为基因组保留不完全处理的多基转录 (GRIPPs).
- 抑制PRMT5或降低pICln导致mRNA,SNRPB和SNRPD3蛋白质被保留在染色质上.
- 染色体捕获的转录表现出较慢的拼接,并且富含被拘留的内子.
- 对snRNP的氨酸甲基化对于它们与染色质和RNA的相互作用至关重要.
结论:
- PRMT5在控制转录处理和拼接完成方面发挥着重要作用.
- 这种由PRMT5进行的处理和拼接控制对于促进特定mRNA类的染色质逃逸和核出口至关重要.
- PRMT5介导的调节影响了拼接与转录输出和基因表达的合.
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