相关实验视频

Updated: Jun 16, 2025

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.4K

通过极化扫描单分子光 (PS-SMF) 显微镜对激子结合 (iCy3) 2二元标记的DNA结构进行DNA呼吸的研究

Jack Maurer1,2, Claire S Albrecht1,2, Patrick Herbert1,2

  • 1Center for Optical, Molecular and Quantum Science, Department of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon 97403.

Proceedings of SPIE--the International Society for Optical Engineering
|August 16, 2024
PubMed
概括

我们开发了一种单分子光方法来测量DNA呼吸动态. 这种技术揭示了蛋白质-DNA复合体组合至关重要的局部DNA构造波动.

关键词:
DNA 呼吸,蛋白质-DNA相互作用激发器合的二进制探头标记着DNA的标签.自由能量是表面的自由能量阶段标记的光子计数 (PTPC)极化选择性单分子光成像成像单分子光学显微镜的光学显微镜.

更多相关视频

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

406
Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

2.1K

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K