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胚胎温度对肌肉circRNA表达和尼罗河鱼体内生长有长期影响

Golam Rbbani¹, Riaz Murshed¹, Prabhugouda Siriyappagouder¹

¹Genomics Division, Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.

Frontiers in cell and developmental biology

|August 16, 2024

概括

胚胎温度显著改变尼罗河鱼的循环RNA (circRNA) 表达,影响长期的肌肉生长. 这些发现揭示了关于水产养殖和气候变化适应的热可塑性的分子见解.

科学领域:

* 发育生物学发育生物学
* 分子遗传学分子遗传学
* 水生动物生理学

背景情况:

*胚胎温度影响脊椎动物的肌肉发育和基因表达.
*循环RNAs (circRNAs) 是关键的调节者,但它们在肌肉生长的热可塑性中的作用还未得到充分研究.
*了解circRNA对温度的反应对于水产养殖和气候变化适应至关重要.

研究的目的:

* 为了研究胚胎温度对尼罗河鱼肌肉中的circRNA转录组的影响.
* 为了确定在不同的热条件下参与肌肉生长可塑性的特定circRNAs.
* 探索circRNAs和myomiRs在热应激反应中的潜在相互作用.

主要方法:

*对尼罗河鱼 (Oreochromis niloticus) 快速肌肉中circRNAs的转录组分析.
*将胚胎暴露在三种不同的温度 (24°C,28°C,32°C) 中,然后在28°C下共同养4个月.
*下一代测序用于识别和量化circRNAs并分析差异表达.

主要成果:

* 在所有温度组中共鉴定出了5,141种独特的circRNA.
*观察到circRNAs的差异表达,例如circNexn,circTTN和circTTN_b的特定circRNAs在对胚胎温度的反应中显示出显著的变化.
*有差异表达的circRNAs与myomiRs表现出潜在的相互作用,这表明它们在肌肉发育中起着调节作用.

结论:

* 胚胎温度显著影响circRNA格局,影响尼罗河鱼的长期肌肉生长可塑性.
*特定的circRNAs被确定可能作为分子标记物用于鱼类肌肉的热适应.
*研究结果提供了关于鱼类对热变化的抵抗力背后的分子机制的关键见解,这与可持续水产养殖和气候变化缓解有关.

关键词:

水产养殖的水产养殖发展性可塑性发展性可塑性肌肉发育 (myogenesis) 是一个过程.没有编码的RNAs.身体生长的体质增长热可塑性热可塑性热可塑性

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