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Updated: Jun 16, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

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基础,糖和骨干修改的合理设计改善了ADAR介导的RNA编辑

Genliang Lu1, Chikdu Shivalila1, Prashant Monian1

  • 1Wave Life Sciences, Cambridge, MA, USA.

Nucleic acids research
|August 16, 2024
PubMed
概括

新的寡核酸设计,称为AIMers,通过与作用于RNA (ADAR) 酶的腺胺酶相互作用来增强RNA编辑. 包括N-3-乌里丁 (N3U) 在内的修改显著提高了体外和体内编辑效率.

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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