干燥的蓝藻细菌在太空飞行中作为高效的等离子体DNA载体
Anne Kakouridis1, Spencer Diamond2, Thomas Eng1
1Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
ACS synthetic biology
|August 16, 2024
概括
菌可以将DNA等离子体运送到太空并返回,而不需要冷藏. 这种方法使合成生物学工具用于太空探索和生物制造的可靠存储和运输成为可能.
科学领域:
- 合成生物学 合成生物学
- 天体生物学 天体生物学
- 微生物学 微生物学
背景情况:
- 生物系统的有效运输对于基于太空的合成生物学和生物制造至关重要.
- 微生物生物生产可以满足人类在资源有限的空间环境中的需求.
- 生物零件和菌株的现场可用性需要可靠的运输解决方案.
研究的目的:
- 开发一种运输DNA等离子体到国际空间站 (ISS) 和返回没有低温存储的方法.
- 建立一个概念验证,以使用自然耐干燥的生物体作为DNA货物载体.
主要方法:
- 使用了菌 *Nostoc punctiforme* PC73102,以其干燥耐受性而闻名.
- 在干燥的 *N. punctiforme* 中将非本地pSCR119等离子体作为细胞内货物运输到国际空间站并返回.
- 通过转化*Escherichia coli*来评估回归后的等离子体完整性和功能.
主要成果:
- 干燥的Nostoc punctiforme成功地将pscr119等离子体运送到太空,并将其返回没有降解.
- 提取的等离子体没有显示DNA损伤或突变.
- 恢复的等离子体是功能性的,并赋予了对大肠杆菌 (Escherichia coli) 的抗性.
结论:
- *Nostoc punctiforme* 作为一个可行的,坚固的宿主,用于在太空中运输DNA质粒.
- 这种方法减少了对生物零件的专用冷链基础设施的需求.
- 为未来基于太空的生物制造和合成生物学应用提供了基础.
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