光通过叶绿体调节广泛的植物替代性多化,通过叶绿体
M Guillermina Kubaczka1, Micaela A Godoy Herz1, Wei-Chun Chen2
1Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular and CONICET-UBA, Instituto de Fisiología, Biología Molecular y Neurociencias, Buenos Aires 1428, Argentina.
概括
光影响大约30%的阿拉比多普西斯基因的替代多化 (APA),需要功能性叶绿体和线粒体活动. 这种规则不同于替代拼接 (AS),因为它不涉及转录延长.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 基因规则 基因规则
背景情况:
- 使者RNA (mRNA) 经历加工,包括裂变和多化 (CPA),成为成熟的.
- 替代多基化 (APA) 通过改变3'未翻译区域的长度来产生mRNA多样性,从而影响基因调节.
- APA在植物发育中起作用,包括种子休眠期和Arabidopsis thaliana*的开花.
研究的目的:
- 为了研究光/黑暗条件对Arabidopsis thaliana的APA的影响.
- 将光介导APA的机制与替代拼接 (AS) 的机制进行比较.
主要方法:
- 在不同的光照条件下分析Arabidopsis thaliana的APA模式.
- 利用光受体的突变系,转录延长因子和基因素脱乙酶.
- 研究质体,线粒体和TOR激酶活性的作用.
主要成果:
- 在大约30%的Arabidopsis基因中,光调节了APA.
- 依赖光的APA需要功能性叶绿体,线粒体和TOR激酶活性,类似于AS.
- 与AS不同,光依赖的APA不依赖转录延长因子或通用染色体放松.
结论:
- 植物中光介导的APA调节涉及叶绿体,与AS机制不同.
- 这一规定似乎与构成性CPA因子丰度的变化有关,这种变化受到质细胞活性的影响.
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