融合细胞的细胞基质距离波动使得快速和连贯的集体迁移成为可能
Marcel Jipp1, Bente D Wagner1, Lisa Egbringhoff1
1University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany.
Cell reports
|August 16, 2024
概括
高度收缩的细胞显示集体迁移速度较慢,细胞与细胞相互作用减少. 短距离的细胞基质相互作用,受到基底性actomyosin的影响,显著影响单层迁移速度.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 机械生物学 机械生物学
背景情况:
- 集体细胞迁移是一个复杂的过程,受细胞-细胞通信,力传递和细胞-基质相互作用的影响.
- 了解控制细胞迁移速度的因素对于发育生物学和疾病研究至关重要.
研究的目的:
- 研究细胞基质距离波动,粘附和引力如何影响汇聚细胞单层的迁移速度和相关性.
- 为了比较野生型 (WT) MDCKII细胞和高度收缩的zonula occludens (ZO) -1/2-贫乏的MDCKII细胞 (dKD) 之间的迁移动态.
主要方法:
- 使用的野生类型 (WT) 和封闭区 (ZO) -1/2-贫乏 (dKD) MDCKII细胞单层.
- 分析了细胞-基质距离波动,细胞-基质粘附和引力的变化.
- 测量细胞单层的平均速度和时间空间相关性.
主要成果:
- 与较少收缩的WT细胞相比,流动的dKD单层表现出平均速度下降.
- dKD细胞显示基质粘附度增加,引力减少,形状更紧,细胞与细胞相互作用减少.
- 基本动因和肌肉素的耗尽表明,短距离细胞基质相互作用,特别是动肌素波动,显著影响迁移速度.
结论:
- 短距离细胞基质相互作用和基底性actomyosin活性在调节集体细胞迁移速度方面发挥着至关重要的作用.
- 在高度收缩的细胞中,细胞与细胞相互作用的减少和基质粘附的增加与单层迁移速度的降低相关.
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