在Caenorhabditis elegans中mTOR抑制剂对衰老的影响的表征
Aihan Zhang1, Gadea Meecham-Garcia1, Chiminh Nguyen Hong1
1Institute of Healthy Ageing, and Research Department of Genetics, Evolution and Environment, University College London, London, UK.
概括
拉帕素可以延长C. elegans的寿命,特别是当在晚年给予时. 在衰老研究中,最佳的剂量和时间是最大化益处和避免毒性的关键.
科学领域:
- 老年学是一门学科.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 拉巴胺素 (mTOR) 途径的机械性标对衰老至关重要.
- 拉巴胺在各种生物体中延长了寿命,但它在C. elegans中的作用是不一致的.
- 在C. elegans中优化Rapamycin的使用需要了解剂量和时间.
研究的目的:
- 为了确定拉帕米辛的最佳剂量和输送时间,以延长C. elegans的寿命.
- 调查拉巴素对C. elegans.与年龄相关的病理和死亡率的影响.
- 为了比较拉帕米辛与其类型,temsirolimus和everolimus的疗效.
主要方法:
- 对成年C. elegans进行脂质体介导的拉巴胺素给药.
- 不同的拉帕米辛剂量和治疗开始时间.
- 评估寿命,发育,生育能力和与年龄有关的病理.
主要成果:
- 在最佳的拉巴胺素剂量下,最大21.9%的寿命延长;在最高剂量时观察到毒性.
- 在晚年,拉巴胺治疗强有力的延长了寿命,相当于~70人年.
- 拉帕米辛抑制了子宫瘤的生长,但没有抑制其他病理,这表明它具有细分抗衰老作用.
结论:
- 拉帕米辛在C. elegans中延长生命的效果取决于剂量和时间.
- 晚年管理是拉帕素中介寿命延长的有效策略,在C. elegans.
- 拉帕米辛表现出细分抗衰老作用,影响特定的病理.
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