3组先天性淋巴细胞在粘膜保护中的背景依赖作用
Leandro P Araujo1, Madeline Edwards1, Koichiro Irie1
1Department of Microbiology and Immunology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Science immunology
|August 16, 2024
概括
第三组先天性淋巴细胞 (ILC3s) 对于肠道平衡和高剂量感染期间的生存至关重要,尽管T细胞产生IL-22. ILC3s在感染早期促进病原体耐受性.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 3组先天性淋巴细胞 (ILC3s) 在粘膜免疫中的作用,特别是与T细胞一起,尚未完全理解.
- 肠道平衡依赖于先天性和适应性免疫细胞之间的复杂相互作用.
- 已知T助手17 (TH17) 细胞和IL-22是粘膜防御中的参与者.
研究的目的:
- 调查ILC3s在内源性T细胞存在时调节肠道免疫力的特定功能.
- 为了确定ILC3s是否对TH17和TH22细胞反应和IL-22产生至关重要.
- 阐明ILC3s和ILC3衍生的IL-22在维持肠上皮质平衡和抵御细菌感染的宿主防御中的作用.
主要方法:
- 利用保留T细胞,TH17细胞和二级淋巴体器官的ILC3缺乏的小鼠.
- 评估了TH17和TH22细胞对开始性和致病性细菌的反应.
- 测量了CD4 T细胞的IL-22产生.
- 在 *Citrobacter rodentium* 感染 (低剂量和高剂量) 期间评估了肠上皮质平衡和宿主存活率.
主要成果:
- ILC3s对于产生TH17和TH22细胞反应以及T细胞衍生的IL-22产生是不可或缺的.
- 尽管存在产生IL-22的T细胞,但ILC3s和ILC3衍生的IL-22对于维持肠上皮质平衡至关重要.
- 虽然T细胞充足,但ILC3缺乏的小鼠清除了低剂量感染,但ILC3s在感染早期促进了病原体耐受性,这对于高剂量挑战期间的生存至关重要.
结论:
- ILC3s在肠道免疫中发挥着上下文依赖的作用,与它们在支持T细胞反应中的作用不同.
- ILC3s对于维持肠道上皮质完整性和宿主存活是不可或缺的,特别是在高感染负载下.
- 这些发现为了解粘膜免疫中ILC3s和TH17细胞的单独功能提供了一个框架.
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