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高灵敏的SERS纳米平台,基于阿普坦和万科米辛,用于检测金黄色细菌及其临床应用

Shanshan Lin¹, Yunsi Zheng², Yanlong Xing³

¹Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, 571199, China; Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, Haikou, 571199, China; Jiangyin Center for Disease Control and Prevention, No. 158 Changjiang Road, Jiangyin, 214431, China.

|August 16, 2024

概括

一种新型的表面增强拉曼散射 (SERS) 纳米平台利用aptamer和vancomycin实现了对金黄色葡萄球菌 (S. aureus) 的高度敏感检测. 这种方法可用于早期检测感染,限量为3.27 CFU/mL.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
分析化学分析化学

背景情况:

黄金葡萄球菌 (S. aureus) 是人类感染的常见原因,从局部性疾病到严重的全身性疾病.
传统的S. aureus检测方法缺乏早期感染诊断所需的灵敏度.
需要先进的平台,能够在感染的早期阶段高度敏感地检测金黄色细菌.

研究的目的:

开发一种基于表面增强拉曼散射 (SERS) 的新型纳米平台,用于高度敏感和早期检测金黄色葡萄球菌 (S. aureus).
为了整合双重识别元素,阿帕特 (Apt) 和万科米辛 (Van),提高S. aureus检测的特异性和效率.
建立一种定量检测方法,用于S. aureus的低检测极限.

主要方法:

制造一个SERS纳米平台使用aptamer结合的Fe3O4磁纳米粒子 (Fe3O4-Apt MNPs) 用于S. aureus丰富.
黄金纳米颗粒 (Au NPs) 的修改与菌素 (Van-Au NPs) 作为SERS探针用于定量检测.
利用磁性分离来分离富化细菌,并分析来自超水体的SERS信号以定量S. aureus.

主要成果:

开发的SERS纳米平台在检测黄金色杆菌方面表现出高灵敏度.
黄金菌的检测极限 (LOD) 确定低至3.27 CFU/mL.
该纳米平台有效地结合了细菌丰富和SERS检测,以准确量化.

结论:

拟议的基于SERS的纳米平台为高度敏感和早期检测特定细菌提供了一个强大的工具,特别是S. aureus.
采用阿普塔默和万科米辛的双重识别策略提高了检测系统的特异性和效率.
这项技术在诊断细菌感染在其初始阶段的临床应用方面具有显著的潜力.

关键词:

阿普塔默和万科米辛的使用方法具有双重识别功能.在S. aureus检测检测系统中.表面增强的拉曼散射 (SERS) 是一种

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