乙醇类型的无氧消化通过刺激直接的物种间电子转移和物种间转移来增强甲生成性能
Shuang Zhang1, Yuanyuan Ren2, Pan Zhao1
1Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Bioresource technology
|August 16, 2024
概括
乙醇预发酵通过促进甲生产和微生物活动,增强了食物废物的无氧消化 (AD). 这种方法刺激了直接的物种间电子转移 (DIET) 和物种间转移 (IHT).
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 食品废弃物的酸化是无氧消化 (AD) 中的一个主要挑战.
- 乙醇预发酵可以减轻酸化,但其对微生物电子转移通路的影响尚不清楚.
- 了解物种间电子转移 (IET) 对于优化AD过程至关重要.
研究的目的:
- 研究乙醇预发酵对食物废弃物无氧消化过程中物种间电子转移 (IET) 的影响.
- 评估乙醇类型AD对甲生产和微生物社区代谢的影响.
- 确定乙醇添加与直接物种间电子转移 (DIET) 和物种间转移 (IHT) 的刺激之间的关系.
主要方法:
- 半连续无氧消化 (AD) 实验使用两种料配置进行:乙醇类型 (COD比率乙醇:酸: propionate:丁酸的5:2:1.5:1.5) 和控制 (0:5:2.5:2.5).
- 有机载荷率 (OLR) 从1.0至5.0gCOD/L/d变化以评估性能.
- 进行了基因丰度分析,以量化对直接物种间电子转移 (DIET) 和物种间转移 (IHT) 相关基因的影响.
主要成果:
- 乙醇类型的AD显著提高了可容忍的最大有机负载率 (OLR) 到6.0gCOD/L/d.
- 在乙醇类型的AD中,甲产量增加了1.2-14.8倍,而OLR为1.0-5.0gCOD/L/d的对照组则增加了1.2-14.8倍.
- 皮拉基因 (与饮食相关) 的丰富度增加了5.6倍,与IHT相关的酶基因被上调.
结论:
- 乙醇预发酵有效地减轻了酸化,并增强了食品废弃物中的甲产量.
- 这个过程刺激了直接的物种间电子转移 (DIET) 和物种间转移 (IHT),从而改善了微生物的新陈代谢.
- 乙醇类型的AD为优化无氧消化效率提供了一个有希望的策略.
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