在生物控制剂Orius laevigatus中,RNA病毒的丰富多样性
Luis Hernández-Pelegrín1, Amador Rodríguez-Gómez2, Ana Belén Abelaira2
1Departamento de Genética e Instituto Universitario de Biotecnología y Biomedicina (BIOTECMED), Universidad de Valencia, 46100 Burjassot, Valencia, Spain.
Journal of invertebrate pathology
|August 16, 2024
概括
这项研究在生物控制关键昆虫Orius laevigatus中发现了27种RNA病毒. 这些病毒,包括大量的solinviviruses和totiviruses,可能会影响昆虫.
科学领域:
- 昆虫学和病毒学.
- 研究昆虫群体内的病毒群体.
背景情况:
- 猎 (Orius laevigatus) 对于生物控制至关重要,但与之相关的病毒的了解很少.
- 越来越多的RNA病毒被发现在Hemiptera昆虫中,影响宿主生物学.
- 下一代测序有助于发现昆虫特异性病毒 (ISV).
研究的目的:
- 为了描述Orius laevigatus的RNA病毒组.
- 在O. laevigatus种群中识别病毒和内源性病毒元素.
- 评估病毒对O. laevigatus生物控制有效性的潜在影响.
主要方法:
- 用下一代测序来分析O. laevigatus的病毒组.
- 在五个不同的昆虫种群中量化了病毒的丰度.
- 进行了基因组分析,以确定非逆转录病毒内源性病毒元素 (nrEVEs).
主要成果:
- 在O. laevigatus.中发现了来自十四个家族的27个RNA病毒的多样化病毒组.
- 23种病毒是O.laevigatus特有的,四种与真菌微生物群有关.
- 在昆虫的基因组中观察到大量的solinvivirus和三个totivirus,以及24个nrEVEs.
结论:
- 这项研究揭示了O. laevigatus.中复杂且丰富的RNA病毒组.
- 确定的病毒和nrEVE表明长期存在宿主病毒关联.
- RNA病毒可能会显著影响O. laevigatus的生物控制性能.
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