抗体库的生成和抗原结合剂的选择
Hyunji Yang1, Jisu Chae1, Hyori Kim2
1Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, South Korea.
Cold Spring Harbor protocols
|August 16, 2024
概括
抗体具有独特的结构优势,并为研究和诊断提供无偏见的B细胞谱. 它们的交叉反应性和明显的帕拉托普形成使它们成为临床前研究和抗体库构建中宝贵的工具.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 兽医科学 兽医科学 兽医科学
背景情况:
- 抗体在研究和诊断中得到广泛使用.
- 它们与哺乳动物表位体具有交叉反应性,有利于在小鼠模型中进行临床前研究.
- 独特的结构特征,比如重链CDR3中的非正规囊,使非传统的帕拉托普形成成为可能.
研究的目的:
- 在研究,诊断和治疗中引入抗体的应用.
- 讨论免疫球蛋白基因的染色体组织.
- 解释塑造抗体谱的多样化机制.
主要方法:
- 对免疫球蛋白基因组织和多样化机制的分析.
- 在抗体变异区域中特有的结构特征的表征.
- 探索使用单个PCR原始组进行肉B细胞受体谱的无偏放大.
主要成果:
- 具有独特的免疫球蛋白基因结构,可促进无偏向的B细胞重组.
- 单一的原始组可以准确地放大重链和轻链VDJ和VJ基因片段.
- 这种无偏见的方法有利于构建菌体显示库和抗原结合剂的in silico选择.
结论:
- 抗体在研究,诊断和治疗开发方面具有明显的优势,因为它们具有独特的曲目和结构性质.
- 抗体基因放大无偏的性质促进了先进的图书馆建设和选择策略.
- 了解免疫球蛋白基因组织和多样化是充分发挥其潜力的关键.
相关概念视频
01:31Hybridoma Technology
14.2K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.2K
01:10Antibody Structure
59.6K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
59.6K