相关实验视频

Updated: May 3, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

准备VCSM13辅助菌体用于显示图书馆再放大和生物面板

Hyunji Yang¹, Jisu Chae¹, Hyori Kim²

¹Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, South Korea.

Cold Spring Harbor protocols

|August 16, 2024

概括

本协议详细说明了VCSM13辅助菌体的制备,这对于放大菌体显示抗体库至关重要. 适当的准备确保了成功的图书馆重新放大和生物分析,以发现抗体.

科学领域:

分子生物学分子生物学
病毒学病毒学
免疫技术是一种免疫技术.

背景情况:

体显示抗体库是抗体发现和工程的关键工具.
对于构建这些库,广泛使用的是 pComb3XSS phagemid 矢量.
这种载体需要辅助菌体来复制和组装菌体颗粒.

研究的目的:

为VCSM13辅助菌素的制备提供详细的协议.
为了确保用户可以有效地生产下游应用的VCSM13辅助菌体.

主要方法:

培养VCSM13辅助菌体的培养.
净化和定位辅助菌素颗粒.
准备好的辅助菌体库存的质量控制.

主要成果:

一种可靠的方法来产生高度VCSM13辅助菌体库存.
为图书馆放大准备的辅助菌体的实用性进行演示.
确认辅助菌体适合进行生物分析实验.

结论:

描述的协议使VCSM13辅助菌体的有效准备成为可能.
适当准备的辅助菌体的可用性对于成功的菌体显示库生产和应用至关重要.

更多相关视频

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

相关概念视频

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...