使用CircProPlus检测蛋白质编码circRNA的最新资源
Xue Gong1,2,3,4, Yunchang Liu2,3, Gengze Wu2,3
1Department of Cardiology, The Sixth Medical Centre, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.
Scientific reports
|August 16, 2024
概括
我们开发了CircProPlus,这是一个自动化管道,用于识别编码蛋白质的圆形RNA (circRNAs). 与CircProPlus集成的CirComPara2有效检测具有编码潜力的circRNA,其性能优于CIRI2.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 编码蛋白质的循环RNA (circRNAs) 是具有显著生理和病理作用的非正规RNA分子.
- 由于当前检测工具的局限性,许多编码蛋白质的circRNA仍然未被识别.
研究的目的:
- 开发CircProPlus,一个自动化的计算管道,用于对翻译的circRNAs进行新的检测.
- 评估CircProPlus在与不同的circRNA检测工具集成时的性能,特别是CIRI2和CirComPara2.
主要方法:
- 开发CircProPlus,用于circRNA检测的增强自动化管道.
- 在CircProPlus框架内对circRNA检测工具 (CIRI2,CirComPara2) 的比较分析.
- 在不同物种和库类型的真实RNA测序数据上进行测试.
主要成果:
- 与以前的方法相比,CircProPlus提供了更好的可访问性,灵活性和生产力.
- 当与CircProPlus一起用于评估circRNA编码潜力时,CirComPara2的表现始终优于CIRI2.
- 该管道在从各种数据集中识别具有蛋白质编码潜力的circRNA方面表现强.
结论:
- CircProPlus是一个强大的工具,用于新检测翻译的circRNAs.
- CirComPara2与CircProPlus的集成增强了具有编码潜力的circRNAs的识别.
- 这种方法通过实现更有效的数据挖掘,推进了circRNA研究领域.
相关概念视频
03:21RNA-seq
9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
02:53CRISPR and crRNAs
16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K