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Updated: Jun 16, 2025

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
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Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Published on: November 1, 2018

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使用石英晶体微平衡的无细胞表达能够快速,动态和无标签地表征膜相互作用蛋白质

Aset Khakimzhan1, Ziane Izri1, Seth Thompson1

  • 1School of Physics and Astronomy, University of Minnesota, Minneapolis, MN, 55455, USA.

Communications biology
|August 16, 2024
PubMed
概括

我们开发了一种新的方法,即在离细胞转录-翻译中,在一个带有消散的石英晶体微平衡中 (TXTL-QCMD),研究膜蛋白如何与细胞膜相互作用. 这种技术允许在没有蛋白质净化或标记的情况下进行动态表征.

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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