来自罗马尼亚的家猫中的Toxoplasma gondii基因型和频率
Adriana Györke1, Anamaria Balea2,3, Silvia Borșan2
1Parasitology and Parasitic Diseases Department, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania. adriana.gyorke@usamvcluj.ro.
BMC veterinary research
|August 16, 2024
概括
罗马尼亚猫群中毒菌感染很普遍,对人类构成重大风险. 这项研究在猫样本中确定了寄生虫的常见欧洲基因型,突出了进一步研究的需要.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 动物传播疾病流行病学
- 传染病的分子流行病学.
背景情况:
- 毒素虫是一种广泛传播的原生动物寄生虫,其复杂的生命周期涉及猫作为最终宿主.
- 猫会分泌传染性卵囊,污染环境,并对包括人类在内的中间宿主构成传播风险.
研究的目的:
- 调查来自罗马尼亚的家猫中Toxoplasma gondii的流行率和遗传多样性.
- 评估在研究地区从猫到人类传播Toxoplasma gondii的风险.
主要方法:
- 使用浮动,PCR,生物测试和血清学测试 (MAT,ImmunoComb) 来分析31只家猫的便,血清和心脏样本.
- 使用nPCR-RFLP针对11个遗传位置的Toxoplasma gondii分离物的基因定型.
- 在罗马尼亚中部和西北部的农村和城市地区从猫身上采集样本.
主要成果:
- 在2/31个便样本中检测到Toxoplasma gondii卵囊.
- 在17/27个血清样本中发现了针对T. gondii的抗体,其标位高达1:384.
- 在7/27的心脏样本中确定了T. gondii的DNA,基因定型揭示了欧洲常见的II型和III型基因型.
结论:
- 这项研究证实了罗马尼亚猫中Toxoplasma gondii感染的高患病率.
- 被感染的猫所产生的卵囊是中介宿主 (包括人类) 感染的重要来源.
- 需要进行进一步的综合性研究,以充分描述在罗马尼亚循环的T. gondii基因型.
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