Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Loss of ASMT Function in Arabidopsis Affects Hormone Pathways and the Ability to Withstand Drought Stress.

International journal of molecular sciences·2026
Same author

Proteogenomic investigation of plant constituents in herbal beverages.

NPJ science of food·2026
Same author

Conflicting Phylogenetic Signals between the Nuclear Ribosomal and Plastome DNA as Evidence for Hybrid Origin of the Tetraploid Member of <i>Salicornia</i> (Amaranthaceae s.l.).

Biochemistry. Biokhimiia·2025
Same author

Seed shattering in common buckwheat (Fagopyrum esculentum): insights from RNA-seq and morphological analysis.

BMC plant biology·2025
Same author

Full-length isoform constructor (FLIC) - a tool for isoform discovery based on long reads.

Bioinformatics (Oxford, England)·2025
Same author

Furanocoumarins in two European species of Heracleum: transcriptomic and metabolomic study.

BMC plant biology·2025

相关实验视频

Updated: Jun 16, 2025

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.2K

转2表达 - - 新型转录组组装管道优化用于基因表达分析.

Aleksandra M Kasianova1,2, Aleksey A Penin1, Mikhail I Schelkunov1,2

  • 1Institute for Information Transmission, Russian Academy of Sciences, Moscow, Russia.

Plant methods
|August 16, 2024
PubMed
概括

Trans2express是新的混合转录组组装管道,为植物生产高质量的基因表达数据. 这种工具有助于描述转录组和分析基因表达,特别是在非模型物种中.

关键词:
从新开始,转录组组合组合.组装管道的组装管道.最长的同位素形式在线工具在线工具.这就是Trans2express的意义.

更多相关视频

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

9.5K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K

相关实验视频

Last Updated: Jun 16, 2025

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.2K
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

9.5K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K

科学领域:

  • 植物基因组学 植物基因组学
  • 转录基因组序列的测序
  • 生物信息学工具 生物信息学工具

背景情况:

  • 对于大型复杂的植物基因组来说,新基因组组装是一个挑战.
  • 转录组组装是一种替代方案,但面临着异型和不均表达等挑战.
  • 现有的转录组件组装器在生产高质量的组件以供下游分析方面扎.

研究的目的:

  • 开发一条可靠的管道,用于新的混合转录组组装.
  • 创建一个基于Web的工具 (Trans2express) 以进行高效的转录组分析.
  • 提高基因表达研究的转录组组件的质量,特别是在非模型植物中.

主要方法:

  • 开发了Trans2express,一个使用rnaSPAdes进行混合转录组组装的管道.
  • 实施后加工过步骤,以提高组装质量.
  • 使用Illumina和牛津纳米孔数据对Arabidopsis thaliana和三个非模型植物物种进行了管道测试.

主要成果:

  • Trans2express实现了高质量的转录组组,其中86.1%的Arabidopsis基因被组装成单个结合体.
  • 组装的转录组与基因表达水平的基因组分析具有高度一致性.
  • 对于模型和非模型植物,有效的差异基因表达分析被证明是有效的.

结论:

  • Trans2express提供了一种新的混合转录组组装协议,旨在为每个基因单个转录恢复.
  • 该协议和网络工具预计将在非模型植物中推进转录组特征和基因表达分析.
  • Trans2express将成为研究基因表达和转录组的植物生物学家的宝贵资源.