微生物同化硫酸减少介导的H2S:在克罗恩病的发展中被忽视的作用
Wanrong Luo1,2, Min Zhao3, Mohammed Dwidar4,5
1Department of Gastroenterology, the First Affiliated Hospital, Sun Yat-Sen University, No.58 Zhongshan Er Road, Room 1209, Guangzhou, 510080, China.
Microbiome
|August 16, 2024
概括
硫化 (H2S) 不平衡与克罗恩病 (CD) 有关. 微生物同化硫酸盐减少 (ASR) 途径,以前未被识别,显著促进了CD患者的肠道H2S产量.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 代谢途径 代谢途径
背景情况:
- 克罗恩氏病 (CD) 与肠道硫化 (H2S) 不平衡,微生物群失调和屏障功能障碍有关.
- 肠道中H2S生成的精确机制及其在CD中的宿主微生物群贡献尚未完全理解.
研究的目的:
- 在CD.的背景下,研究人类宿主和肠道微生物群的硫性潜力.
- 阐明特定微生物代谢途径在H2S生产中的作用.
主要方法:
- 从未接受过治疗的CD队列中分析便元基因组和活检元转录基因组数据.
- 使用工程E. coli突变体缺乏同化硫酸盐减少 (ASR) 途径的实验验证.
- 大肠炎的小鼠模型,以评估改变H2S生产的影响.
主要成果:
- 癌症患者的宿主H2S基因表达减少,微生物H2S生产基因增加.
- 微生物ASR途径,涉及硫酸盐还原酶和L-氨酸合成酶,被确定为肠道中主要的H2S贡献者.
- 细菌中的ASR缺乏影响了细菌的硫生成能力,结肠细胞活力和粘素硫化,导致小鼠的结肠炎.
- 肠道微生物群通过ASR降解硫多聚糖,产生H2S,这表明硫多聚糖在结肠炎中的作用.
结论:
- 微生物ASR途径是人类肠道中一个重要的,以前被忽视的内源H2S生产者.
- 了解微生物的硫代谢,特别是ASR途径,为管理CD提供了潜在的治疗点.
- 食中的硫多糖类可能会影响H2S的产生和结肠炎,因此应谨慎使用它们作为食品添加剂.
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