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模块化基于芯片的纳米SFC-MS用于超快速分离

Chris Weise¹, Martin Schirmer², Matthias Polack¹

¹University Leipzig, Linnestrasse 3, Leipzig 04103, Germany.

Analytical chemistry

|August 17, 2024

概括

这项研究引入了一种小型化的超临界流体染色学质谱仪装置,用于快速的奇拉分离. 基于芯片的系统实现了高速,可重复的结果,采样量和移动阶段数量最小.

科学领域:

分析化学分析化学
分离科学分离科学
质谱测量质量谱测量

背景情况:

超临界流体色谱 (SFC) 是一种强大的分离技术.
分析仪器的小型化在速度和灵敏度方面提供了优势.
将SFC与质谱学 (MS) 结合起来,可以提高检测能力.

研究的目的:

开发一个小型的,基于芯片的设备,用于超临界流体染色学与质谱学 (SFC-MS) 结合.
为了使用开发的nanoSFC系统实现高速和可重复的合分离.
在转移到大气压MS接口时保持色谱完整性.

主要方法:

开发一个模块化,基于芯片的纳米SFC系统,配有颗粒包装的纳米孔柱.
微流体组件的集成,用于精确的样品负载 (picoliter体积) 和逆压控制.
使用限制性发射器和最小后列体积 (16nL) 来防止减压和稀释.

主要成果:

开发的设备成功地在不到80秒的时间内完成了高速合分离.
在染色分离方面实现了高可重现性.
该系统有效地将分析物转移到MS接口,而不会损害染色学完整性.

结论:

微型化,基于芯片的nanoSFC-MS设备对快速和可复制的性分离是有效的.
该设计克服了与移动相解压和分析剂稀释相关的挑战.
这种方法为高吞吐量和抗分离分析提供了一个有前途的平台.

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