在低压TEM成像中DIY适应SEM
Zecca Piero Antonio1, Protasoni Marina1, Reguzzoni Marcella1
1DIMIT, Department of Medicine and Technological Innovation, University of Insubria, Varese, Italy.
Microscopy research and technique
|August 17, 2024
概括
这项研究比较了扫描电子显微镜 (SEM) 和传输电子显微镜 (TEM),发现TEM提供了更高的清晰度. 然而,修改后的SEM系统在较低的电压下提供TEM类成像,大大减少了敏感材料的样品损伤.
科学领域:
- 材料科学 材料科学 材料科学
- 生物学 生物学 生物学
- 显微镜技术 显微镜技术
背景情况:
- 高质量的电子显微镜对于详细的样本分析至关重要.
- 电子显微镜中的高能光束可能会损害敏感样本.
- 扫描电子显微镜 (SEM) 和传输电子显微镜 (TEM) 是具有不同优势的关键技术.
研究的目的:
- 为了比较传统的TEM和修改后的SEM系统之间的图像质量,噪声水平和样本保存.
- 为了评估使用传输电子转换配件修改SEM的有效性.
- 评估较低的加速电压对成像敏感材料的影响.
主要方法:
- 传统的TEM和一个修改后的SEM系统的比较分析,配备了传输电子转换配件.
- 图像噪声水平和纹理特征的定量评估 (,对比度,不相似性,同质性,能量,相关性).
- 使用修改后的SEM系统在低加速电压下对各种样品进行成像.
主要成果:
- 传统的TEM产生的图像清晰度更高,噪音水平明显降低.
- 修改后的SEM系统在非常低的电压下产生高质量的图像,这对于敏感样品至关重要.
- 与修改后的SEM相比,TEM图像呈现出较低的和更高的同质性,表明质地更光滑.
结论:
- 对于需要最高清晰度和最低噪音的研究,TEM仍然是首选的方法.
- 修改后的SEM系统提供了一种多功能替代方案,提供类似于TEM的成像,减少样品损伤.
- 选择合适的电子显微镜技术取决于样品的灵敏度和所需的细节水平.
更多相关视频
相关概念视频
Transmission Electron Microscopy
5.4K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.4K
Overview of Electron Microscopy
8.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.6K
Scanning Electron Microscopy
4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
4.2K
Overview of Microscopy Techniques
9.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.8K
Preparation of Samples for Electron Microscopy
5.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.4K
Electron Microscope Tomography and Single-particle Reconstruction
2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K


