全转录组测序揭示了骨髓细胞的增殖和分化过程中的ceRNA调节网络
Liangchao Xiao1, Jiahui Chen1, Xueying He1
1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China.
Poultry science
|August 17, 2024
概括
这项研究揭示了关键基因和非编码RNA网络,调节了Shitou的肌肉发育. 了解这些分子机制可以提高这种大型品种的肉类生产效率.
科学领域:
- 基因组学和分子生物学
- 动物科学和动物繁殖
背景情况:
- 什图是最大的肉类品种,是研究肌肉发育的宝贵模型.
- 提高肉类生产效率需要了解肌肉生长的遗传调节.
研究的目的:
- 识别关键基因, lncRNAs,circRNAs和miRNAs,这些基因参与了什图的肌肉发育.
- 构建调节网络,包括控制骨肌肉生长和纤维形成的ceRNA网络.
- 为了阐明背后的分子机制,Shitou的优越的肉类生产特征.
主要方法:
- 胚胎腿部肌肉的整体转录基因分析.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 使用生物信息学工具 (Miranda,TargetScan,miRDB) 识别调控网络 (lncRNA-mRNA,circRNA-mRNA,miRNA-mRNA,ceRNA网络) 的方法.
- 使用蛋白质-蛋白质相互作用 (PPI) 分析和Cytoscape构建一个lncRNA/circRNA-miRNA-mRNA ceRNA网络.
主要成果:
- 确定了847个差异表达的基因 (DEG),244个差异表达的lncRNA (DEL),37个差异表达的circRNA (DEC) 和84个差异表达的miRNA (DEM).
- 丰富分析揭示了肌肉结构发育,基于actin丝的过程和细胞骨通路中的重要作用.
- 确定了包括FoxO,AMPK,Wnt和信号在内的关键信号通路.
- 构建了复杂的调节网络,其中ceRNA网络显著丰富于actin细胞骨组织,突出显示了非编码RNA在肌肉纤维形成中的作用.
结论:
- 这项研究提供了一个全面的转录调节网络,用于的肌肉发育.
- 这些发现提供了关于什图肌肉生长和肉类生产的分子机制的见解.
- 这项工作为未来的研究奠定了基础,通过遗传调节优化肉类生产特征.
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