重新审视豆类培养素:结构组织和碳水化合物结合性质
Vinicius J S Osterne1, Gilles De Sloover1, Els J M Van Damme1
1Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Proeftuinstraat 86, 9000, Ghent, Belgium.
Carbohydrate research
|August 17, 2024
概括
豆类学菌素具有相同的结构,但在碳水化合物结合方面有所不同. 本综述详细介绍了它们的结构-功能关系,用于糖生物学和治疗学的应用.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- 豆类学蛋白是蛋白质,可以结合碳水化合物.
- 它们共享保存的结构特征,包括形成碳水化合物识别域 (CRD) 的β三明治折叠.
- 尽管结构相似,但它们对不同糖具有不同的特异性.
研究的目的:
- 审查豆类培养素的结构方面.
- 为了检查它们的碳水化合物结合特性和特定的连接体.
- 了解潜在应用的结构结合关系.
主要方法:
- 保存β三明治折叠的结构分析.
- 分析参与碳水化合物结合的关键残留物 (键,疏水性相互作用,范德瓦尔斯力).
- 基于特异性组 (例如,曼诺/葡萄糖,银糖,福克,酸) 的lectins的分类.
主要成果:
- 豆类培养素具有保留的三级结构,但具有可变的四级结构和结合特异性.
- 在CRD子结构和残留物中的变化决定了特定的连接体识别.
- 莱克丁可以根据首选的配体进行分组,例如曼诺斯/葡萄糖,N-乙-d-银胺/银糖,N-乙-d-葡萄糖胺,l-糖和α-2,3酸.
结论:
- 了解豆类培养素的结构和结合对于阐明它们的生物作用至关重要.
- 这些见解有助于开发用于糖生物学,诊断和治疗的豆类讲蛋白.
- 保存的折叠与可变的CRD允许多样化的碳水化合物识别.
相关概念视频
Chemistry of Carbohydrates
72.1K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
72.1K
Cell Adhesion in Plants
2.7K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.7K
Membrane Carbohydrates
5.3K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
5.3K
What are Carbohydrates?
170.5K
Overview
170.5K
Protein and Protein Structure
79.2K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
79.2K
Selectins
3.3K
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.3K


