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CD122是一种激活标志物,可确保T细胞在远位细胞中的适当增殖

Ming Geng¹, Yi Cao¹, Kang Li¹

¹State Key Laboratory of Estuarine and Coastal Research, School of Life Sciences, East China Normal University, Shanghai, 200241, China.

Fish & shellfish immunology

|August 17, 2024

概括

研究人员开发了一种新型的单克隆抗体用于 CD122,将其识别为T细胞激活标记物. 这一发现揭示了CD122的存在.

科学领域:

免疫学免疫学免疫学
鱼类生物学鱼类生物学
分子生物学分子生物学

背景情况:

介素-2受体 (IL-2R) 亚单元CD122在适应性免疫中起作用.
关于teleost CD122功能的研究,特别是在T细胞水平上,由于缺乏特定抗体而受到限制.

研究的目的:

描述尼罗河鱼CD122的特征,并开发一种特定的单克隆抗体 (mAb).
研究CD122在鱼T细胞激活和增殖中的作用.

主要方法:

尼罗河鱼的生物信息分析和克隆 CD122.2.
通过免疫和杂交瘤技术生产一种小鼠抗虫CD122单克隆抗体 (mAb).
在免疫组织和T细胞子集中检测CD122表达,使用开发的mAb.
通过mAb阻断实验评估CD122在T细胞信号通路 (MAPK/Erk,mTORC1) 和增殖中的作用.

主要成果:

成功开发了一种特定的抗蒂拉皮亚CD122 mAb.
CD122在免疫组织中广泛分布,在感染或T细胞激活时被上调.
在激活过程中,CD122的表达在T细胞上增加,这表明其作为小病中T细胞激活标记物的潜力.
阻止CD122 mAb抑制了MAPK/Erk和mTORC1通路的激活和T细胞的增殖.

结论:

CD122作为尼罗河鱼中可靠的T细胞激活标志物.
CD122在鱼T细胞增殖中起着至关重要的作用.
这项研究提高了对鱼类T细胞反应和适应性免疫的演变的理解.

关键词:

适应性免疫是一种适应性免疫.CD122 是一个CD122 一个单克隆抗体的抗体.色色素尼洛蒂库斯 (Oreochromis niloticus) 是一种多彩的植物.激活T细胞的激活过程

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