催化酶对As (III) 和As (V) 的不同反应:在价值效应下潜在的分子机制
Tingting Wang1, Mingyang Jing2, Shaoyang Hu1
1School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, PR China.
The Science of the total environment
|August 17, 2024
概括
(As) 暴露,特别是来自水和水中的,对健康有害. 这项研究表明,化物 (As (III)) 和化物 (As (V)) 损害了抗氧化酶催化酶 (CAT) 的结构和功能,而As (III) 更强大.
科学领域:
- 环境毒理学环境毒理学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- (As) 是一种广泛存在的食品污染物,主要存在于水和水中,导致病.
- 有限的毒理学数据存在于酸盐 (As (III)) 和酸盐 (As (V)) 对抗氧化酶催化酶 (CAT) 的分子水平影响.
研究的目的:
- 在分子水平上研究As (III) 和As (V) 与CAT的相互作用机制.
- 阐明这些物种如何影响CAT的结构和生理功能.
主要方法:
- 酶活性检测试验测定了酶活性.
- 多光谱技术 (UV-Vis,光) 的使用
- 异热定位热量计 (ITC) 是一种热量计.
- 计算模拟的计算模拟.
主要成果:
- As (III) 和As (V) 诱导了CAT的结构变化,包括蛋白质骨架放松,二次结构转换和光变化.
- 与As (V) 相比,As (III) 对CAT的结构和功能的影响更为明显.
- 结合相互作用涉及结合和疏水力,对As (III) 和As (V) 确定了特定的残留接触.
结论:
- As (III) 和As (V) 通过改变其分子结构来抑制CAT活性.
- 由于 (III) 对CAT的完整性和功能构成了比As (V) 更大的威胁.
- 这些发现有助于更好地了解与饮食中暴露相关的健康风险.
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