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通过双目标RAA-LFD试验快速视觉检测Helicobacter pylori和vacA亚型

Sijie Yin¹, Yanghe Liu², Xinyi Yang³

¹Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, China; Department of Laboratory Medicine, Yangzhou HongquanHospital, Yangzhou, China.

Clinica chimica acta; international journal of clinical chemistry

|August 17, 2024

概括

一种新的双目标复合酶辅助放大 (RAA) 试验与侧向流量测量棒 (LFD) 检测快速识别了Helicobacter pylori (H. pylori) 和其 vacA病毒性基因. 这项为期30分钟的测试提供了一个可靠的,设备独立的工具,用于H. pylori检测和基因定型.

科学领域:

分子诊断学分子诊断学
微生物学微生物学
医疗技术医疗技术医学技术

背景情况:

杆菌 (H. pylori) 感染影响全球超过50%的人口,显著增加胃癌的风险.
H. pylori 的致病性与像 vacA 基因这样的毒性因素有关.
准确的H. pylori鉴定和基因型定型对于有效的临床管理和疾病控制至关重要.

研究的目的:

开发一种快速,可视和准确的双目标测定,用于H. pylori检测和vacA基因型定型.
通过重组酶辅助放大 (RAA) 和侧向流量计 (LFD) 方法来评估试验的性能.

主要方法:

开发了一种双目标RAA-LFD试验,用于检测H. pylori 16srRNA和ureA基因,以及vacA m1/m2等位基因.
使用复合酶辅助放大 (RAA) 在37°C的双目标放大.
采用横向流量计 (LFD) 用于放大产品的视觉检测.

主要成果:

该试验在不到30分钟的时间内实现了双目标放大和视觉检测.
证明了高特异性,没有针对常见细菌病原体的交叉反应.
临床评估显示,对H. pylori检测和vacA基因型定型 (kappa=1.00) 的定量聚合酶链反应 (qPCR) 完全一致.

结论:

开发的RAA-LFD测定是一种快速,可靠和设备独立的方法,用于H. pylori检测和vacA基因型定型.
该试验的临床验证支持其对有效的H. pylori流行控制和相关疾病的向治疗的潜力.

关键词:

在Helicobacter Pylori的研究中,侧向流量测量棒横向流量测量棒再组合酶辅助的放大.vacA m1 的时间.空地 m2 平方米

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