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一种促进 Dendrobium huoshanense protocorm-like bodies 的 GLP-1 分泌的酸性多糖:结构验证和活动探索

Zhen-Zi Shang¹, Hui-Yu Ye¹, Xin Gao¹

¹School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, People's Republic of China.

International journal of biological macromolecules

|August 17, 2024

概括

来自Dendrobium huoshanense的一种新型多糖化合物h-DHP增强了类似葡萄糖-1 (GLP-1) 的分泌. 这促进了葡萄糖平衡,并可能通过增加内源GLP-1水平来帮助体重管理.

科学领域:

药理学药理学是指药理学的学科.
生物化学生物化学
内分泌学在内分泌学.

背景情况:

类似葡萄糖类-1 (GLP-1) 是一种关键激素,调节葡萄糖平衡和体重.
增强内源GLP-1分泌为代谢障碍提供治疗潜力.
内分泌L细胞是GLP-1分泌的主要来源.

研究的目的:

单独和描述一种新型化合物,可以增强GLP-1分泌.
通过新型化合物研究GLP-1增强背后的机制.
评估该化合物在调节葡萄糖平衡和体重方面的潜力.

主要方法:

来自Dendrobium huoshanense的新型多糖体 (h-DHP) 的隔离和结构识别.
在小鼠体内研究,以评估h-DHP注射后的血GLP-1水平.
使用STC-1细胞进行体外研究,以确认h-DHP对GLP-1分泌的影响.
药理上抑制信号通路 (腺酸环酶,脂酶C) 以阐明机制.

主要成果:

发现了一种新型的酸性多糖,h-DHP (1.38 × 10^5 Da),由银河糖,葡萄糖,阿拉比诺酸和葡萄糖酸组成.
在小鼠中,h-DHP显著增加了血GLP-1水平,在STC-1细胞中显著增加了细胞外GLP-1.
h-DHP的GLP-1增强作用涉及cAMP信号和细胞内的增加,由腺酸环酶和脂酶C介导.

结论:

h-DHP有效地增强了来自肠内分泌细胞的内源GLP-1分泌.
该机制涉及cAMP介导的信号通路.
作为改善血糖控制和控制体重的治疗剂,h-DHP显得有前途.

关键词:

丹德罗比亚姆 (Dendrobium huoshanenseense) 是一种植物.在GLP-1分泌物中.多糖是一种多糖化合物.

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