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Establishment of a Clinic-based Biorepository
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使用已建立的生物存储库来解决新出现的研究问题:一个可行性研究
Lente J S Lerink1,2, Christopher W Sutton3, Henny G Otten
1Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK.
Clinical proteomics
|August 17, 2024
概括
长期存储的临床血清样本适用于蛋白质和代谢途径分析. 这一发现支持未来的移植研究和罕见疾病研究.
科学领域:
- 生物分子分析.
- 移植研究 移植研究
- 这是生物银行.
背景情况:
- 移植研究可以从蛋白质组学和代谢组学中受益.
- 通过生物库可以获得捐赠者样本,但缺乏用于长期研究的受体样本.
- 临床血清存储库具有潜力,但由于处理不均,需要对-omics应用进行验证.
研究的目的:
- 评估长期存储的临床血清样本是否适合用于蛋白质和代谢途径分析.
- 为了比较临床血清样本与protocolized生物库样本.
主要方法:
- 采用了基于质谱的猎枪蛋白质组学和代谢组学.
- 分析了来自PROCARE联盟的临床血清样本.
- 同时对QUOD生物库的样本进行了分析.
主要成果:
- 在两种样本类型中确定了800多种化合物和600种蛋白质.
- 163种内源代谢物在临床和生物银行样本中都是常见的.
- 综合途径分析成功地绘制了蛋白质组和代谢组数据,丰富了79条途径.
结论:
- 长期存储的临床血清样本可用于定性蛋白质和代谢途径分析.
- 这证实了现有临床样本用于未来研究的有效性.
- 影响包括推进生物医学研究,长期结果研究和罕见事件调查.
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