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相关概念视频

ATP Synthase: Structure01:18

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ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
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Protein Transport to the Stroma

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Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
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RNA Structure01:19

RNA Structure

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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相关实验视频

Updated: Jun 16, 2025

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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人类TIP60复合体的结构

Ke Chen1, Li Wang2, Zishuo Yu1

  • 1Fudan University Shanghai Cancer Center, Institutes of Biomedical Sciences, State Key Laboratory of Genetic Engineering and Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College of Fudan University, Shanghai, 200032, China.

Nature communications
|August 17, 2024
PubMed
概括
此摘要是机器生成的。

人类的TIP60复合体

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X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
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相关实验视频

Last Updated: Jun 16, 2025

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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 生物化学 生物化学

背景情况:

  • 哺乳动物TIP60是一种关键的酶,参与转录,DNA修复和细胞周期控制.
  • 它具有基因素乙化和基因素二聚物交换活动.
  • 了解它的结构是解读其多样化的细胞功能的关键.

研究的目的:

  • 为了确定人体TIP60复合体的高分辨率冷电子显微镜结构.
  • 阐明TIP60.0.的分子架构和子单位组织.
  • 为了解TIP60如何与核细胞相互作用提供见解.

主要方法:

  • 低温电子显微镜 (cryo-EM) 在3.2-Å分辨率.
  • 人类TIP60复合体的结构改进,包括核心和TRRAP模块.
  • 综合体内的蛋白质-蛋白质相互作用的分析.

主要成果:

  • EP400蛋白质作为一个中心支架,集成电机,ARP和TRRAP模块.
  • 特定的蛋白质相互作用定义了电机模块 (RUVBL1-RUVBL2六合体,EP400 ATPase,YL1) 和ARP模块 (ACTL6A-ACTB异构体,DMAP1,EPC1) 的组合.
  • ARP模块显示与电机模块的稳定关联,但与TRRAP模块的灵活绑定,与核心和TRRAP模块之间放置的未被绑定的核酶体.

结论:

  • 这项研究揭示了人类TIP60复合体的详细分子结构.
  • 这些发现为TIP60复合体与核细胞相互作用提供了结构性的见解.
  • 这项工作为了解TIP60在各种细胞过程中的作用奠定了基础.