相关实验视频

Updated: Jun 16, 2025

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Published on: December 6, 2013

丸细胞衍生条件介质支持人类胚胎干细胞的生殖细胞分化

Fatemeh Akyash^1,2,3, Reza Aflatoonian⁴, Ehsan Farashahi-Yazd^1,3

¹Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

|August 18, 2024

概括

人类胚胎干细胞 (hESCs) 可以在体外使用丸细胞受条件介质 (TCCM) 分化为生殖细胞. 这种方法有助于研究体育生殖和不孕症的原因.

科学领域:

干细胞生物学干细胞生物学
发育生物学是发展生物学.
生殖生物学生殖生物学

背景情况:

研究人类生殖细胞发育提出了伦理和技术上的挑战.
人类胚胎干细胞 (hESCs) 为体外生育发育研究提供了有价值的替代方案.
了解游戏生成对于解决不孕症至关重要.

研究的目的:

在体外将hESC分化为生殖细胞.
调查丸细胞衍生条件介质 (TCCM) 在支持生殖细胞分化方面的疗效.
建立一个模型来研究游戏生殖和不孕症.

主要方法:

人类胚胎干细胞 (Yazd2; 46XY) 被培养成胚胎体 (EB).
EB被暴露在不同的介质条件下,包括TCCM和自发差异化 (SD) 控制.
基因表达 (RT-qPCR) 和免疫染 (IF) 在4日,7日和14日进行,以评估差异化.

主要成果:

与SD组相比,TCCM组表现出增加的游戏生成基因表达.
在所有组的分化细胞中,在体外体育生成 (IVG) 中证实了免疫化.
基因表达分析揭示了成功的分化标记.

结论:

人类TCCM是体外男性和女性生殖细胞发育的自然利基.
需要进一步的研究来确定TCCM中的特定因素.
这种方法为生殖生物学研究提供了一个有前途的工具.

关键词:

有条件的中介.不同化的差异化细菌细胞细菌细胞人类胚胎干细胞

更多相关视频

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Published on: October 7, 2020

相关概念视频

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...