从沙巴的中分离出来的低致病性禽流感H9N2病毒的序列分析和分子特征
S A Shohaimi1, B L Leow1, F F Mohd Yusop1
1Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.
Tropical biomedicine
|August 18, 2024
概括
马来西亚沙巴的一种新的低致病性禽流感 (LPAI) H9N2菌株显示,由于特定的遗传标记,可能会传播给人类. 建议加强监测和生物安全,以减轻对家禽和公众健康的风险.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 公共卫生 公共卫生
背景情况:
- 低致病性禽流感 (LPAI) H9N2对家禽健康和潜在的公共健康构成重大威胁.
- H9N2在马来西亚半岛是本土的,但在2022年8月在沙巴发生了令人担忧的疫情,导致 brojler的高死亡率.
研究的目的:
- 为了基因鉴定H9N2病毒的特征,该病毒从沙巴的一个肉疫情中分离出来.
- 分析H9N2血凝素 (HA) 基因的遗传变异和遗传关系.
- 评估H9N2分离物对家禽和人类健康的潜在风险.
主要方法:
- 测序和分析H9N2分离体的血凝素 (HA) 基因.
- 对HA分裂部位和受体结合性质的氨基酸分析.
- 遗传学分析以确定分离物的进化血统和起源.
主要成果:
- 沙巴H9N2分离体与2018年中国H9N2病毒具有98%的HA基因相似性.
- 该HA裂变部位具有特征性的LPAI动机,但位于226位的氨基酸 (Leucine) 表明潜在的哺乳动物受体结合.
- 遗传学分析将该分离物置于类h9.4.2.5 (Y280血统) 内,但与之前的马来西亚分离物不同,表明了新的引入来源.
结论:
- 来自萨巴的H9N2分离物具有遗传特征,包括潜在的哺乳动物受体结合,这引起了公众健康的关注.
- 独特的遗传学聚类表明H9N2在马来西亚的新型引入.
- 持续监测家禽和实施农场生物安全措施对于控制H9N2传播和减轻相关风险至关重要.
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