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Updated: Jun 16, 2025

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在冷电子断层扫描中对螺旋丝的亚断层扫描平均值的协议

Xiaojie Zhang1, Julia Mahamid2

  • 1Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Baden-Württemberg, Germany.

STAR protocols
|August 18, 2024
PubMed
概括

本研究介绍了使用子图片平均值的螺旋丝的高分辨率3D成像协议. 该方法使得像病毒核囊体这样的宏分子结构的详细可视化,有助于细胞组织研究.

关键词:
生物物理学的生物物理.化电磁波是一种冷电磁波.显微镜的使用方法

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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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