在强心研究中对状况和DNA甲基化进行了全表观基因组研究
Wil Lieberman-Cribbin1, Arce Domingo-Relloso2, Ronald A Glabonjat1
1Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York, NY, USA.
Environment international
|August 18, 2024
概括
(Se) 是必不可少的,但其对健康的影响尚不清楚. 这项研究在美国印第安人成年人中发现了最小的DNA甲基化变化,这表明其他因素影响了Se相关的健康结果.
科学领域:
- 环境表观遗传学环境表观遗传学
- 营养毒理学 营养毒理学
- 心血管疾病流行病学
背景情况:
- (Se) 是一种重要的微量元素,具有复杂的剂量依赖的健康影响.
- 了解表观遗传机制,特别是基因甲基化 (DNAm) 的理解,是Se对健康的影响的关键.
- 强心研究 (SHS) 美国印第安人成年人队列为研究这些关系提供了一个独特的人口.
研究的目的:
- 研究尿液Se水平与全基因组DNA甲基化 (DNAm) 模式之间的关联.
- 为了确定与Se暴露相关的特定差异甲基化位置 (CpG位点).
- 在心血管疾病 (CVD) 风险的背景下探索Se的表观遗传影响.
主要方法:
- 用ICP-MS测量了1357名美国印第安人成年人的尿液Se度.
- 使用Illumina MethylationEPIC BeadChip (850K Array) 评估了全血DNA甲基化情况.
- 用强大的线性回归和弹性网模型进行了表观基因组范围的关联分析.
主要成果:
- 在多重比较校正后,五个差异甲基化位置 (DMP) 与尿中的Se水平显著相关 (FDR p值:0.10).
- 顶部高甲基化DMP (cg00163554) 被注释为DIP2C基因,该基因参与转录调节.
- 弹性网模型确定了425个DMP,其中包括在回归分析中发现的3个,将Se与DIP2C,MAP4K2和GPIHBP1基因中的甲基化变化联系起来.
结论:
- 尿Se与美国印第安人成年人的微妙DNA甲基化变化有关.
- 这些发现表明,Se的健康影响可能是由DNA甲基化之外的机制调解的.
- 需要进一步的研究来探索其他生物标志物,潜在的关系和疾病关联.
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