在葡萄藤中通过高通量测序识别一种新型米托病毒
Malyaj R Prajapati1, Damini Diksha1, Pooja Thapa1
1Advanced Center for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India, 110012.
概括
一种新型病毒,葡萄藤线粒病毒1 (GMV1),在葡萄藤中使用高通量测序发现. 这种线粒病毒编码了一个完整的RNA-依赖RNA聚合酶,使其与许多其他已知的线粒病毒区别开来.
科学领域:
- 病毒学 病毒学
- 植物病理学 植物病理学
- 分子生物学分子生物学
背景情况:
- 植物转录组测序通常会产生病毒RNA读数.
- 这些读数可以组装起来,重建病毒基因组.
- 同时的病毒RNA分离在植物RNA制备过程中很常见.
研究的目的:
- 在葡萄树根茎中识别和描述新型RNA病毒.
- 为了研究一个新发现的米托病毒的基因组特征.
主要方法:
- 葡萄藤根茎RNA的高通量测序 (HTS).葡萄藤根茎RNA的高通量测序.
- 来自HTS数据的病毒RNA序列的组合.
- 病毒特异性RT-PCR测定用于确认.
- 病毒基因组的BLASTx分析和遗传学分析.
主要成果:
- 鉴定了一个假定的新型线粒病毒,葡萄藤线粒病毒1 (GMV1).
- GMV1基因组RNA编码了一个完整的2,496核酸开放读取框架 (ORF),包括一个依赖RNA的RNA聚合酶 (RdRp) 域.
- 33.18-56.75%的序列相同性与现有的线粒病毒;家族遗传学分析将GMV1置于一个不同的类.
结论:
- 葡萄藤真菌病毒1 (GMV1) 是一个新发现的物种,属于Mitoviridae家族的Unuamitovirus属.
- 转基因病毒1感染葡萄树内的真菌线粒体.
- 与大多数已知的线粒体病毒不同,GMV1编码了一个完整的功能RdRp,与真菌和植物线粒体遗传密码一致.
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