阿迪波涅克丁受体1通过腺单酸激活蛋白激酶/E-cadherin通路调节子宫内膜受体
Bolor-Erdene Sarankhuu1, Hye Jin Jeon1, Da-Un Jeong1
1Priority Research Center, Myunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea.
Molecular medicine reports
|August 19, 2024
概括
阿迪波涅丁受体1 (ADIPOR1) 对子宫内膜受体性至关重要,影响胚胎植入. 它对AMP激活蛋白激酶 (AMPK) /E-cadherin通路的调节对于生育治疗至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 蜂信号传输是如何进行的
背景情况:
- 子宫内膜受容性对于成功怀孕至关重要.
- 阿迪波涅丁及其受体1 (ADIPOR1) 与生殖功能有关.
- 需要阐明ADIPOR1在子宫内膜受体性中的特定作用.
研究的目的:
- 调查ADIPOR1在人类子宫内膜受体性中的调节作用.
- 探索将ADIPOR1与子宫内膜功能联系起来的分子机制.
- 评估ADIPOR1通路在改善生育治疗方面的潜力.
主要方法:
- 利用RL95-2和AN3CA细胞系与体外JAr球状附着模型来评估子宫内膜受体性.
- 使用短发针RNA (shRNA) 和lentiviral载体来降低ADIPOR1的表达.
- 进行了反转录定量PCR,免疫块分析,并使用了特定的AMPK调节剂 (dorsomorphin,5-aminoimidazole-4-carboxamide ribonucleotide).
主要成果:
- 与非受体AN3CA细胞相比,ADIPOR1表达在中间分泌阶段子宫内膜和受体RL95-2细胞中较高.
- 减少ADIPOR1的表达导致减少E-cadherin的表达,并损害了体外子宫内膜接受能力.
- ADIPOR1通路调节了AMP激活蛋白激酶 (AMPK) 活性,这反过来又影响了E-cadherin表达和子宫内膜受体性.
结论:
- ADIPOR1/AMPK/E-cadherin信号轴在调节子宫内膜受容性方面发挥着至关重要的作用.
- 准ADIPOR1可能为增强生育治疗和改善妊娠结果提供一种新的策略.
相关概念视频
GPCRs Regulate Adenylyl Cylase Activity
5.4K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.4K
Hormonal Regulation of the Menstrual Cycle
320
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
320
cAMP-dependent Protein Kinase Pathways
6.2K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.2K
Regulation of Food Intake
205
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
205
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K


