积极准对氧反应敏感的多功能细胞,用于癌症的协同化疗
Haile Fentahun Darge1,2,3, Kefyalew Dagnew Addisu1,4, Hsieh-Chih Tsai1,5,6
1Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
新的迪戈辛修饰聚合物微粒提供向的癌症治疗. 这些氧化还原反应药物递送系统增强了多克索鲁比的疗效,并减少了癌细胞中的副作用.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 癌症治疗方法 癌症治疗方法
背景情况:
- 响应刺激的聚合物微粒对药物输送有希望,提高疗效和减少副作用.
- 有针对性的输送系统对于改善癌症治疗结果至关重要.
研究的目的:
- 合成和表征一种新型的毒素修饰的,氧化还原反应的,二硫化物结合的多 (乙烯基醇-b-多 (乳糖-co-甘油酸)) 共聚物 (Bi-Dig-PEG-PLGA) -S2).
- 评估其作为多功能药物递送载体的潜力,用于在癌细胞中向和控制地释放多克索鲁比 (DOX).
主要方法:
- 自组装Bi (Dig-PEG-PLGA) -S2成球形的小粒.
- 微粒大小,泽塔潜力,血清和体稳定性的表征.
- 药物负载能力 (DLC) 和封装效率 (EE) 的评估.
- 在氧化还原条件下 (GSH和H2O2) 释放多克索鲁比 (DOX) 的体外评估.
- 在HeLa细胞系中进行体外研究,以评估细胞内积累和亡效应.
主要成果:
- -PEG-PLGA) -S2形成了具有良好的稳定性的均球形 (58.36 ± 0.78 nm).
- 实现了高药物载荷能力 (6.26%) 和封装效率 (83.23%).
- 观察到具有氧反应的DOX释放,在GSH和H2O2的存在下显著释放.
- DOX@Bi(Dig-PEG-PLGA) -S2在HeLa细胞中显示出增强的细胞内积累和亡效应.
结论:
- 合成的Bi ((Dig-PEG-PLGA) -S2微粒对向和受控的多克索鲁比输送是有效的.
- 迪戈辛部分增强细胞吸收和化学敏感化,改善抗癌效应.
- 这些发现突显了Bi(Dig-PEG-PLGA) -S2作为癌症治疗的多功能纳米载体的潜力.
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