在人类血清中基于LC-MS/MS的分支链和芳香氨基酸的生物分析
Tianyi Wang1, Yalian Zhang2, Luan Jia2
1Department of GCP, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, P.R. China.
Bioanalysis
|August 19, 2024
概括
一种新的LC-MS/MS方法准确地测量血清中的分支链氨基酸 (BCAA) 和芳香氨基酸 (AAA). 这种快速的技术有助于诊断肝损伤,并建立健康人口的参考间隔.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 分析化学 分析化学
背景情况:
- 分链氨基酸 (BCAA) 和芳香氨基酸 (AAA) 被认为是肝病中的潜在生物标志物.
- 准确有效地测量这些氨基酸对于诊断和管理肝脏疾病至关重要.
研究的目的:
- 开发和验证一种快速的LC-MS/MS方法,用于同时量化血清BCAA和AAA.
- 在中国健康成年人群中建立这些氨基酸的参考间隔.
- 评估该方法是否适用于肝损伤诊断的常规临床使用.
主要方法:
- 血清样本经历了一步的蛋白质沉以准备.
- 液体染色学-双重质谱法 (LC-MS/MS) 用于分析.
- 分析运行时间优化为每个样本4分钟.
主要成果:
- 开发的LC-MS/MS方法表现出极好的线性,R平方值超过0.9969.
- 准确度是令人满意的,范围从94.44%到107.75%.
- 精度很高,变化系数在0.10%至5.90%之间.
结论:
- 经过验证的LC-MS/MS方法适用于高通量临床应用.
- 这种方法可以作为肝损伤的有价值的辅助诊断工具.
- 建立的参考间隔将有助于解释中国人群中的患者结果.
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