转录组和蛋白质组的变化是由人类病原体Leishmania major中转录调节器Maf1的过度表达引发的
Luis A Rivera-Rivas1, Luis E Florencio-Martínez1, Gabriela Romero-Meza2
1Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico.
概括
Maf1蛋白抑制了Leishmania major中的RNA聚合酶III转录,影响了tRNA,rRNA和snRNA水平. 过度表达显示了Maf1.
科学领域:
- 分子生物学分子生物学
- 寄生虫学的寄生虫学
- 基因组学就是基因组学.
背景情况:
- Maf1蛋白是酵母中RNA聚合酶III (RNAPIII) 转录的已知抑制剂.
- 它在真核生物中的功能是多样化的,但对像Leishmania major这样的原生虫寄生虫的知识是有限的.
研究的目的:
- 为了调查Maf1在Leishmania大病中的作用.
- 描述Maf1过度表达对RNAP III活性和全球基因表达的影响.
主要方法:
- 产生一种超表达Maf1的Leishmania主要细胞系.
- 用于转录基因分析的RNA测序.
- 无标签的定量质谱仪用于蛋白质组分析.
- 基因本体学和KEGG通路丰富分析.
主要成果:
- Maf1过度表达显著降低了tRNA,5SrRNA和U4 snRNA的丰度,证实了RNAP III的调节.
- 全球分析确定了1082个差异表达的转录和205个差异表达的蛋白质.
- 丰富分析将Maf1与转录,细胞循环,新陈代谢,自和细胞骨调节联系起来.
结论:
- Maf1在Leishmania major中起着保守的调节作用,影响转录,新陈代谢和细胞循环过程.
- 这项研究表明,Maf1参与了mRNA转录后控制,这是一个以前没有在其他生物体中描述过的新功能.
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