多基因多态性与NKG2A谱系有关,并影响淋巴细胞表型和功能
Jean-Benoît Le Luduec1, Theodota Kontopoulos1, M Kazim Panjwani1
1Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY.
Blood advances
|August 19, 2024
概括
杀手细胞中单核酸多态性 (SNP) 类似甲酸蛋白受体C1 (NKG2A) 影响NKG2A表达和NK细胞功能. HLA-C表位也预测HLA-E表达,影响NK细胞反应和疾病预后.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞和分子免疫学 细胞和分子免疫学
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 在NK和T细胞上的CD94/NKG2A受体综合体在结合HLA-E时调解抑制信号.
- NKG2A的表达水平影响NK细胞的反应能力和整体免疫功能.
- HLA-E表达是由HLAI类信号和的可用性调节的.
研究的目的:
- 识别与免疫细胞NKG2A表达相关的单核酸多态 (SNPs).
- 为了研究丰度和HLA-E表达之间的关系.
- 确定NKG2ASNP和HLA-C表位的NK细胞表型和功能的预测值.
主要方法:
- 分析与NKG2A表达相关的杀手细胞甲酸样受体C1 (KLR C1) SNPs.
- 调查KLR C1,KLR2 (NKG2C) 和KLRK1 (NKG2D) 多态之间的链接不平衡.
- NKG2A表面表达与NK细胞响应能力和频率的相关性.
- 分析可用性和HLA-E表达水平之间的关系.
- 识别HLA-C表位素作为HLA-ABC和HLA-E表达的预测标记.
主要成果:
- 几个KLR C1 (NKG2A) SNP与NKG2A在NK,CD8+和Vγ9/Vδ2+T细胞上的表达有关.
- 在KLRRC2 (NKG2C) 和KLRK1 (NKG2D) 中的多态性与NKG2A表面密度和频率有关.
- 的可用性与HLA-E表达水平有很强的相关性.
- HLA-C1表位与高HLA-E表达相关,而HLA-C2表位与低HLA-E表达相关,独立于HLA-E全型.
- HLA-E表达会影响NK细胞的抑制,但不会影响NKG2A介导的NK教育.
结论:
- NKG2A SNPs和HLA-C表位体作为NK细胞表型和功能的重要预测标记.
- 这些遗传因素会影响先天免疫反应和NK细胞的抑制性.
- NKG2A SNPs和HLA-C表位值得作为高HLA-E表达的疾病的预后标志物进行评估.
更多相关视频
05:24Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
Published on: February 21, 2021
4.1K
07:58qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
Published on: March 6, 2019
8.6K
相关概念视频
Diversity of Antigen Receptors
550
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
550
T Cell Activation and Clonal Selection
693
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
693
Cytotoxic T Cells-mediated Immune Response
861
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
861
Epistasis
46.6K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
46.6K
B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
Epistasis Analysis
4.9K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.9K
